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Structured Review

Procell Inc hs683 human glioma cell line
( A ) The qRT-PCR assays validated the siRNA knockdown effect. ( B ) The results of CCK-8 assay in <t>HS683</t> cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.
Hs683 Human Glioma Cell Line, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hs683+human+glioma+cell+line/pmc11208487-90-1-10?v=Procell+Inc
Average 90 stars, based on 1 article reviews
hs683 human glioma cell line - by Bioz Stars, 2026-07
90/100 stars

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1) Product Images from "Stemness subtypes in lower-grade glioma with prognostic biomarkers, tumor microenvironment, and treatment response"

Article Title: Stemness subtypes in lower-grade glioma with prognostic biomarkers, tumor microenvironment, and treatment response

Journal: Scientific Reports

doi: 10.1038/s41598-024-65717-7

( A ) The qRT-PCR assays validated the siRNA knockdown effect. ( B ) The results of CCK-8 assay in HS683 cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.
Figure Legend Snippet: ( A ) The qRT-PCR assays validated the siRNA knockdown effect. ( B ) The results of CCK-8 assay in HS683 cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Techniques Used: Quantitative RT-PCR, Knockdown, CCK-8 Assay

Scratch wound healing assessment of HS683 cell lines following treatment with siRNA or negative control (NC) targeting four hub genes: ( A ) CDCA8, ( B ) DLGAP5, ( C ) ORC1 and ( D ) SMC4. Transwell examination of LGG cell lines treated with siRNA or negative control (NC) to target four hub genes: ( E ) CDCA8, ( F ) DLGAP5, ( G ) ORC1 and ( H ) SMC4. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.
Figure Legend Snippet: Scratch wound healing assessment of HS683 cell lines following treatment with siRNA or negative control (NC) targeting four hub genes: ( A ) CDCA8, ( B ) DLGAP5, ( C ) ORC1 and ( D ) SMC4. Transwell examination of LGG cell lines treated with siRNA or negative control (NC) to target four hub genes: ( E ) CDCA8, ( F ) DLGAP5, ( G ) ORC1 and ( H ) SMC4. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Techniques Used: Negative Control

The results of CCK-8 assay in HS683 cell lines. ( A ) SMC4, ( B ) DLGAP5, CDCA8 and ( D ) ORC1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.
Figure Legend Snippet: The results of CCK-8 assay in HS683 cell lines. ( A ) SMC4, ( B ) DLGAP5, CDCA8 and ( D ) ORC1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Techniques Used: CCK-8 Assay



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Image Search Results


Association between EIF4E and PBK and patient survival. The co-expression relationship between EIF4E and PBK was performed by the cBioPortal online platform (A). The expression of EIF4E was downregulated after PBK knockdown in HS683 (B). GEPIA online platform was used to perform the expression of EIF4E in glioma and its effect on survival. EIF4E mRNA expression in GBM compared to normal brain tissues (C) in which normal samples are marked in gray and GBM samples are marked in red. High EIF4E expression decreases both overall survival (D) and disease-free survival (E).

Journal: Annals of Medicine and Surgery

Article Title: Unraveling the role of PBK in glioblastoma: from molecular mechanisms to therapeutic targets

doi: 10.1097/MS9.0000000000002708

Figure Lengend Snippet: Association between EIF4E and PBK and patient survival. The co-expression relationship between EIF4E and PBK was performed by the cBioPortal online platform (A). The expression of EIF4E was downregulated after PBK knockdown in HS683 (B). GEPIA online platform was used to perform the expression of EIF4E in glioma and its effect on survival. EIF4E mRNA expression in GBM compared to normal brain tissues (C) in which normal samples are marked in gray and GBM samples are marked in red. High EIF4E expression decreases both overall survival (D) and disease-free survival (E).

Article Snippet: The human glioma cell line HS683 was obtained from the American Type Culture Collection (ATCC) and cultured according to ATCC instructions in DMEM medium with 10% FBS in a 5% CO 2 incubator at 37°C. shRNA sequences for PBK knockdown and cell line establishment were based on a previous study .

Techniques: Expressing, Knockdown

( A ) The qRT-PCR assays validated the siRNA knockdown effect. ( B ) The results of CCK-8 assay in HS683 cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Journal: Scientific Reports

Article Title: Stemness subtypes in lower-grade glioma with prognostic biomarkers, tumor microenvironment, and treatment response

doi: 10.1038/s41598-024-65717-7

Figure Lengend Snippet: ( A ) The qRT-PCR assays validated the siRNA knockdown effect. ( B ) The results of CCK-8 assay in HS683 cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Article Snippet: The HS683 human glioma cell line was sourced from Wuhan Procell Life Science and Technology Co., Ltd. (Procell, China).

Techniques: Quantitative RT-PCR, Knockdown, CCK-8 Assay

Scratch wound healing assessment of HS683 cell lines following treatment with siRNA or negative control (NC) targeting four hub genes: ( A ) CDCA8, ( B ) DLGAP5, ( C ) ORC1 and ( D ) SMC4. Transwell examination of LGG cell lines treated with siRNA or negative control (NC) to target four hub genes: ( E ) CDCA8, ( F ) DLGAP5, ( G ) ORC1 and ( H ) SMC4. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Journal: Scientific Reports

Article Title: Stemness subtypes in lower-grade glioma with prognostic biomarkers, tumor microenvironment, and treatment response

doi: 10.1038/s41598-024-65717-7

Figure Lengend Snippet: Scratch wound healing assessment of HS683 cell lines following treatment with siRNA or negative control (NC) targeting four hub genes: ( A ) CDCA8, ( B ) DLGAP5, ( C ) ORC1 and ( D ) SMC4. Transwell examination of LGG cell lines treated with siRNA or negative control (NC) to target four hub genes: ( E ) CDCA8, ( F ) DLGAP5, ( G ) ORC1 and ( H ) SMC4. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Article Snippet: The HS683 human glioma cell line was sourced from Wuhan Procell Life Science and Technology Co., Ltd. (Procell, China).

Techniques: Negative Control

The results of CCK-8 assay in HS683 cell lines. ( A ) SMC4, ( B ) DLGAP5, CDCA8 and ( D ) ORC1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Journal: Scientific Reports

Article Title: Stemness subtypes in lower-grade glioma with prognostic biomarkers, tumor microenvironment, and treatment response

doi: 10.1038/s41598-024-65717-7

Figure Lengend Snippet: The results of CCK-8 assay in HS683 cell lines. ( A ) SMC4, ( B ) DLGAP5, CDCA8 and ( D ) ORC1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Article Snippet: The HS683 human glioma cell line was sourced from Wuhan Procell Life Science and Technology Co., Ltd. (Procell, China).

Techniques: CCK-8 Assay